Ambar Rukmini


The effect of water-in-oil (w/o) microemulsion containing ascorbic acid on photo-oxidative stability of virgin coconut oil (VCO) was investigated. To optimize the formulation, w/o microemulsions were prepared using hydrophilic lipophilic balance (HLB) concept, consisted of ternary nonionic surfactants having low, medium, and high HLB values. From this optimum HLB number, the ratio of water, surfactant and oil was determined to obtain microemulsion areas. After optimization of the microemulsion system, the storage at room temperature, and the extreme condition were conducted for testing the microemulsion stability. Then, the stable formulas were used for delivering ascorbic acid. The solubility and photo-oxidative stability of ascorbic acid microemulsion were evaluated. The optimum formula of ascorbic acid microemulsion was applied into VCO at various concentrations (0, 1, 5, and 10% w/w). Samples were subjected to photo-oxidation under fluorescent light exposure of 4000 lux for up to 8 hours. Peroxide values (PVs) and p-anisidine values (p-AnVs) of the samples were measured at 1 hour interval. The results indicated that w/o microemulsion could be formed on the HLB number of 7. W/o microemulsion containing 75% oils need surfactant concentrations of ≥ 4.5 part of water, and if containing 77.78% oils, the surfactant concentration must ≥ 5.5 part of water. These microemulsions remained stable during storage at room temperature, even after centrifugation, but did not tolerate at high temperature. The maximum solubility of ascorbic acid in w/o microemulsion was 1%. Ascorbic acid microemulsion resistant to photo-oxidation and effective inhibits that reaction in VCO. This study confirmed that w/o microemulsion can act as ascorbic acid delivery system to disperse into VCO for inhibiting its’ quality deterioration due to photo-oxidation.


inhibitory effect; ascorbic acid microemulsion; photo-oxidation; virgin coconut oil

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Anwar F, Chatha SAS dan Hussain AI. 2007. Assessment of oxidative deterioration of soybean oil at ambient and sunlight storage. Grasas Y Aceites 58(4):390-395.

AOCS. 2004. Official Methods and Recommended Practices of the AOCS, Fifth edition. American Oil Chemists’ Society, Champaign, Illinois.

Bodannes RS dan Chan PC. 1979. Ascorbic acid as a scavenger of oxygen singlet. Febs Letters 105(2):195-196.

Cho YH, Kim S, Bae EK, Mok CK dan Park J. 2008. Formulation of a cosurfactant-free o/w microemulsion using nonionic surfactant mixtures. Journal of Food Science 73(3):E115-E121.

Constantinides PP dan Scalart JP. 1997. Formulation and physical characterization of water-in-oil microemulsions containing long- versus medium-chain glycerides. International Journal of Pharmacology 158:57-68.

Fanun M. 2009. Microemulsions : Properties and Applications. CRC Press, New York.

Flanagan J dan Singh H. 2006. Microemulsion : a potensial delivery system for bioactives in food. Critical Review of Food Science and Nutrition 46:221-237.

Flanagan J, Kortegaard K, Pinder DN, Rades T dan Singh H. 2006. Solubilisation of soybean oil in microemulsions using various surfactants. Food Hydrocolloids 20:253-260.

Garti N, Spernath A, Aserin A dan Lutz R. 2005. Nano-sized self-assemblies of nonionic surfactants as solubilization reservoirs and microreactors for food systems. Soft Matter 1:206-218.

Kim HJ, Hahm TS dan Min DB. 2007. Hydroperoxide as a prooxidant in the oxidative stability of soybean oil. Journal of the American Oil Chemists’ Society 84(3):349-355.

Kruglyakov PM. 2000. Hydrophile-Lipophile Balance of Surfactants and Solid Particles: Physicochemical Aspects and Applications, First edition. Elsevier Science B.V,.Amsterdam, Netherlands.

Lee KH,. Jung MY dan Kim SY. 1997. Quenching mechanism and kinetics of ascorbyl palmitate for the reduction of the photosensitized oxidation of oils. Journal of the American Oil Chemists’ Society 74(9):1053-1057.

Li P, Ghosh A, Wagner RF, Krill S, Joshi YM dan Serajuddin ATM. 2005. Effect of combined use of nonionic surfactant on formation of oil-in-water microemulsions. International Journal of Pharmacology 288:27-34.

List GR, Wang T dan Shukla VKS. 2005. Storage, Handling and Tarnsport of Oils and Fats dalam Bailey’s Industrial Oil and Fat Products, 6th ed., vol. 5. John Wiley & Sons, Inc., Hoboken, New Jersey.

Marina AM, Man YBC and Nazimah SAH (2009) Chemical Properties of Virgin Coconut Oil. Journal of the American Oil Chemists’ Society 86:301-307.

Mosca M, Ceglie A dan Ambrosone L. 2008. Antioxidant dispersions in emulsified olive oils. Food Research International 41:201-207.

Nostro PL. 1997. Supramolecular Aggregates from Vitamin C Derivatives: Structure and Properties. 97014/index.htm [23 May 2009]

Osborn HT and Akoh CC. 2004. Effect of Emulsifier Type, Droplet Size, and Oil Concentration on Lipid Oxidation in Structured Lipid-Based Oil-in-Water Emulsion. Food Chemistry 84:451-456.

Pakpayat N, Nielloud F, Fortune R, Tourne-Peteilh C, Villarreal A, Grillo I dan Bataille B. 2009. Formulation of ascorbic acid microemulsions with alkyl polyglycosides. European Journal of Pharmacology 72:444-452.

Patel MR, Patel RB, Parikh JR, Bhatt KK dan Kundawala AJ. 2007. Microemulsion : as novel drug delivery vehicle. Lates Reviews vol. 5 issue 6 (

Pilemand C. 2002. Surfactants : Their Abilities and Important Physico-Chemical Properties. Arbejdsmiljøinstituttet, København.

Rahmani M and Csallany AS (1998) Role of Minor Constituents in the Photo-oxidation of Virgin Olive Oil. Journal of the American Oil Chemists’ Society 75(7):837-843

Shahidi F and Zhong Y. 2005. Lipid Oxidation: Measurement Methods in Bailey’s Industrial Oil and Fat Products, 6th ed., vol.5. John Wiley & Sons, Inc., Hoboken, New Jersey.

Wanasundara PKJPD and Shahidi F. 2005. Antioxidants: Science, Technology, and Applications in Bailey’s Industrial Oil and Fat Products, 6th ed., vol.5. John Wiley & Sons, Inc., Hoboken, New Jersey.

Yamamoto Y. 2001. Role of active oxygen species and antioxidants in photoaging. Journal of Dermatological Science 27(1):S1-S4.


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